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LDHA elisa kit product blog

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Posted on 2015-07-13 11:12:01 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Lactate Dehydrogenase A; LDHA; LDHA elisa kit;
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The Rat LDHA ldha (Catalog #MBS704326) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704326 ELISA Kit recognizes Rat (Rattus, General) LDHA.

The LDHA ldha product has the following accession number(s) (GI #126722813) (NCBI Accession #NP_001075746.1) (Uniprot Accession #P13491). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat L-Lactate Dehydrogenase, L-LDH ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) LDHA.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to L-LDH. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for L-LDH and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain L-LDH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of L-LDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates
Detection Range: 39 mU/ml-2500 mU/ml
Sensitivity: 9.7 mU/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Biosynthesis/Metabolism
Protein Biological Process 2: glyconeogenesis and glycometabolism
Protein Biological Process 3: Glycolysis. Rat L-Lactate Dehydrogenase, L-LDH ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. LDHA also interacts with the following gene(s): ALDOA, GOT1, GPI, LDHD, PKLR, PKM2. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.
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