LAMA1 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; Laminin; LAMA1; LAMA1 elisa kit;
The Human LAMA1 lama3 (Catalog #MBS175954) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175954 ELISA Kit recognizes Human LAMA1.The LAMA1 lama3 product has the following accession number(s) (Uniprot Accession #B0YJ33). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Laminin PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) (Human Laminin PicoKine ELISA Kit standard curve )
Principle of the assay: human Laminin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A polyclonal antibody from rabbit specific for Laminin has been precoated onto 96-well plates. Standards(from human fibroblasts) and test samples are added to the wells, a biotinylated detection polyclonal antibody from rabbit specific for Laminin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Laminin amount of sample captured in plate.
Background: Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2.1 Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation.2 The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids.1 The human laminin A chain is at locus 18p11.3.3 The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide.2 The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31.4 The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.5 The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.6 The standard product used in this kit is isolated from plasma.
Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type: Sandwich
Detection Range: 156pg/ml-10,000pg/ml
Sensitivity: < 10pg/ml. Human Laminin PicoKine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.