KGF 2 recombinant protein product blog
Tags: Recombinant Protein; Keratinocyte Growth Factor-2; KGF 2 recombinant protein; KGF 2;
The KGF 2 fgf10 (Catalog #MBS142180) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The KGF 2 fgf10 product has the following accession number(s) (GI #4758360) (NCBI Accession #NP_004456.1) (Uniprot Accession #O15520). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MLGQDMVSPE ATNSSSSSFS SPSSAGRHVR SYNHLQGDVR WRKLFSFTKY FLKIEKNGKV SGTKKENCPY SILEITSVEI GVVAVKAINS NYYLAMNKKG KLYGSKEFNN DCKLKERIEE NGYNTYASFN WQHNGRQMYV ALNGKGAPRR GQKTRRKNTS AHFLPMVVHS.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Keratinocyte Growth Factor-2 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized FGF-10 in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.79 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of FGF-10 as a Reference Standard.
Biological Activity: The ED50, calculated by the dose-dependant stimulation of FGF receptors by BaF3 indicator cells (measured by 3H-thymidine uptake) is < 0.5 ng/ml, corresponding to a specific activity of 2x106units/mg. KGF 2 also interacts with the following gene(s): BMP4, FGFR1, FGFR2, FGFR4, GLI3, PTF1A, SHH, SPRY2, TBX4, TBX5. The following patways have been known to be associated with this gene. Brain, Intestine, Kidney, Mixed, Muscle, Trachea, Uterus tissues are correlated with this protein. Cardiovascular Abnormalities, Cleft Palate, Disease Models, Animal, Heart Defects, Congenital, Inflammation, Lung Diseases, Lung Neoplasms, Prostatic Diseases, Prostatic Neoplasms, Skin Diseases are some of the diseases may be linked to Recombinant Human Keratinocyte Growth Factor-2.