Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow INH-B elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-CD161 antibody blog
 • CD83 elisa kit blog
 • CCDC171 sirna blog
 • anti-mRpL11 antibody blog
 • Griffonia lectin blog
 • FAM98B shrna blog
 • VPS28 sirna blog
 • BHMT sirna blog
 • PM20D2 sirna blog
 • anti-Digoxin antibody blog
 • ERLEC1 sirna blog
 • NDUFA3 sirna blog
 • PIBF1 elisa kit blog
 • anti-FZD3 antibody blog
 • anti-Estriol 3 antibody blog
 • Adenine Sulfate nucleotide blog
 • anti-Staphylococcus Enterotoxin Type D antibody blog
 • GLOD4 sirna blog
 • anti-MHC CLASS II RT1B antibody blog
 • A4GALT shrna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Protochlorophyllide reductase B, chloroplastic (PORB) Recombinant Protein
 • transforming growth factor, beta-induced, 68kDa (TGFBI) ELISA Kit
 • Sal-like protein 3, SALL3 ELISA Kit
 • ADNP Antibody
 • Protein Kinase B Beta (PKBb) ELISA Kit
 • PSMA4 Antibody
 • Calcium-activated chloride channel regulator family member 3... ELISA Kit
 • Leptin Quadruple Antagonist Recombinant Protein
 • Mucin-1, MUC1 ELISA Kit
 • Goat F(ab')2 Anti-Rabbit IgG (H+L chain specific) Secondary Antibody
 • Shc (SHC1) Antibody
 • Interleukin 6 (IL-6) ELISA Kit
 • ACYP1 Peptide
 • Intercellular Adhesion Molecule 1 (ICAM-1) ELISA Kit
 downarrow more ...

INH-B elisa kit product blog

Posted on 2013-08-20 15:05:09 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rat ELISA Kit; INH-B elisa kit; INH-B; inhibin B, INH-B;
The Rat INH-B n/a (Catalog #MBS704128) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704128 ELISA Kit recognizes Rat (Rattus, General) INH-B.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat inhibin B, INH-B ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) INH-B.

Introduction: Inhibin is a peptide that is an inhibitor of FSH synthesis and secretion, and participates in the regulation of the menstrual cycle. Inhibin contains an alpha and beta subunit linked by disulfide bonds. Two forms of inhibin differ in their beta subunits (An or B), while their alpha subunits are identical. Inhibin belongs to the transforming growth factor-beta (TGF-beta) superfamily. In women, FSH stimulates the secretion of inhibin from the granulosa cells of the ovarian follicles in ovary. In turn, inhibin suppresses FSH.Inhibin secretion is diminished by GnRH, and enhanced by insulin-like growth factor-1 (IGF-1). Inhibins have been defined based on their activity of suppressing pituitary gonadotropin secretion. Thus, the serum concentrations of Inhibin B and FSH are inversely correlated, and at low serum levels of Inhibin B, FSH concentration goes up. Inhibin B serum concentration increases gradually in the follicular phase to a broad peak at 7 days prior to the LH surge, and may constitute the limiting factor for the duration of the inter-cycle FSH rise. A decrease and a second peak follow this peak one-day past the LH surge. Most of the serum Inhibin B concentration originates from large follicles, since such follicles secrete 10 fold higher concentrations than do the small follicles. Inhibin B concentrations drop to a very low concentration at the luteal phase, and is undetectable during pregnancy.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated INH-B and antibody preparation specific for INH-B, and incubated. Then substrate solutions are added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of INH-B in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Samples: Serum, plasma, tissue homogenates
Detection Range: 1 pg/ml-200 pg/ml
Sensitivity: 0.5 pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Rat inhibin B, INH-B ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions