Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow IL8 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • TTG-lgA elisa kit blog
 • IgG (H&L), F(ab)'2 Fragment secondary antibody blog
 • CMPK2 blocking peptide blog
 • RSPO3 blocking peptide blog
 • APPBP2 sirna blog
 • ZNF429 blocking peptide blog
 • CASPL2 peptide blog
 • PFKL sirna blog
 • anti-Thrombin antibody blog
 • PROX2 sirna blog
 • GSH-PX elisa kit blog
 • anti-CCL1 antibody blog
 • OLFM1 cdna clone blog
 • ARMET peptide blog
 • FGF20 blocking peptide blog
 • ANO6 sirna blog
 • IRX3 peptide blog
 • IAA17 recombinant protein blog
 • A/Brisbane/10/06 antigen blog
 • Tissue Array, BioAssay Bladder transitional cell carcinoma, grade I-III tissue array blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Interferon Beta (IFN-beta) ELISA Kit
 • fumarylacetoacetate hydrolase (fumarylacetoacetase) (FAH) ELISA Kit
 • Macrophage Inflammatory protein-3 alpha (mMIP 3alpha) Recombinant Protein
 • TRIM5 Antibody
 • VEGFR-2 (CD309) (VEGFR-2 (CD309)) Antibody
 • Tet1 Antibody
 • 2,3-Bisphosphoglycerate Mutase (BPGM) Recombinant Protein
 • PINP (Procollagen Type I N-Terminal Propeptide) CLIA Kit
 • Bauhinia purpurea Lectin Lectin
 • Paraoxonase 2 (PON2) Recombinant Protein
 • Parainfluenza type 1 + 3 Antibody
 • Tissue Inhibitors Of Metalloproteinase 2 (TIMP2) Recombinant Protein
 • CD70 Blocking Peptide
 downarrow more ...

IL8 elisa kit product blog

Posted on 2014-09-28 17:25:35 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Interleukin 8; IL8; Dog ELISA Kit; IL8 elisa kit;
The Dog IL8 cxcl8 (Catalog #MBS700607) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700607 ELISA Kit recognizes Dog (Canine, General) IL8.

The IL8 cxcl8 product has the following accession number(s) (GI #55741690) (NCBI Accession #NP_001003200.1) (Uniprot Accession #P41324). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Dog Interleukin-8, IL-8 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) IL8.

Introduction: Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. There are more receptors of the surface membrane capable to bind IL-8. The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Primary function of IL-8 is the induction of chemotaxis in its target cells (e.g. neutrophil granulocytes). In neutrophils series of cell-physiological responses required for migration and its target function phagocytosis are also induced like increase of intracellular Ca2+, exocytosis (e.g. histamine release), respiratory burst. IL-8 can be secreted by any cells with toll-like receptors which are involved in the innate immune response. IL-8\'s primary function is to recruit neutrophils to phagocytose the antigen which trigger the antigen pattern toll-like receptors. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-8. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-8. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions