|The Dog IL8 cxcl8 (Catalog #MBS700607) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700607 ELISA Kit recognizes Dog (Canine, General) IL8.
The IL8 cxcl8 product has the following accession number(s) (GI #55741690) (NCBI Accession #NP_001003200.1) (Uniprot Accession #P41324). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Dog Interleukin-8, IL-8 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. There are more receptors of the surface membrane capable to bind IL-8. The protein encoded by this gene is a member of the CXC chemokine family. This chemokine is one of the major mediators of the inflammatory response. This chemokine is secreted by several cell types. It functions as a chemoattractant, and is also a potent angiogenic factor. Primary function of IL-8 is the induction of chemotaxis in its target cells (e.g. neutrophil granulocytes). In neutrophils series of cell-physiological responses required for migration and its target function phagocytosis are also induced like increase of intracellular Ca2+, exocytosis (e.g. histamine release), respiratory burst. IL-8 can be secreted by any cells with toll-like receptors which are involved in the innate immune response. IL-8\'s primary function is to recruit neutrophils to phagocytose the antigen which trigger the antigen pattern toll-like receptors. When first encountering an antigen, the primary cells to encounter it are the macrophages who phagocytose the particle. Upon processing, they release chemokines to signal other immune cells to come in to the site of inflammation. IL-8 is one such chemokine. It serves as a chemical signal that attracts neutrophils at the site of inflammation, and therefore is also known as Neutrophil Chemotactic Factor.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-8. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-8. in the samples is then determined by comparing the O.D. of the samples to the standard curve.