IL10 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; IL-10; IL10; IL10 elisa kit;
The Human IL10 il10 (Catalog #MBS175968) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175968 ELISA Kit recognizes Human IL10.The IL10 il10 product has the following accession number(s) (GI #124292) (NCBI Accession #P22301.1) (Uniprot Accession #P22301). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human IL-10 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Picokine Human HSPG2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human HSPG2 with a 96-well strip plate that is pre-coated with antibody specific for HSPG2. The detection antibody is a biotinylated antibody specific for HSPG2. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Human HSPG2 with immunogen: Expression system for standard: NSO, Immunogen sequence: E3687-S4391. The kit is analytically validated with ready to use reagents. To measure Human HSPG2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human HSPG2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Human HSPG2 in the sample
Background/Introduction: Perlecan (PLC) also known as HSPG2, is a protein that in humans is encoded by the HSPG2 gene. It is mapped to 1p36.12. Perlecan is highly conserved across species and the available data indicate that it has evolved from ancient ancestors by gene duplication and exonshuffling. Perlecan is a key component of the vascular extracellular matrix, here it interacts with a variety of other matrix components and helps to maintain the endothelial barrier function. It is a potent inhibitor of smooth muscle cell proliferation and is thus thought to help maintain vascular homeostasis. Perlecan can also promote growth factor (e.g., FGF2) activity and thus stimulate endothelial growth and re-generation.
Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
Detection Range: 0.624ng/ml-40ng/ml
Sensitivity: <0.05ng/ml.