|The IL-8 cxcl8 (Catalog #MBS590014) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase.
The IL-8 cxcl8 product has the following accession number(s) (GI #10834978) (NCBI Accession #NP_000575.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Interleukin-8 (IL-8) with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the assay: This IL-8 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for IL-8. Standards or samples are then added to the appropriate microtiter plate wells and incubated. IL-8, if present, will bind and become immobilized by the antibody pre-coated on the wells. The microtiter plate wells are thoroughly washed to remove unbound IL-8 and other components of the sample. In order to quantify the amount of IL-8 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated monoclonal antibody specific for IL-8 is added to each well to "sandwich" the IL-8 immobilized during the first incubation. The microtiter plate then undergoes a second incubation. The wells are thoroughly washed to remove all unbound HRP-conjugated antibodies and a TMB (3,3\'5,5\' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-8 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450nm +/- 2nm. In order to measure the concentration of IL-8 in the samples, this kit contains two calibration diluents (Calibrator Diluent I for serum/plasma testing and Calibrator Diluent II for cell culture supernatant/ urine testing). According to the testing system, the provided standard is diluted (2-fold) with the appropriate Calibrator Diluent and assayed at the same time as the samples. This allows the operator to produce a standard curve of Optical Density (O.D.) versus IL-8 concentration (pg/mL). The concentration of IL-8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Cell Culture Supernatant, serum, plasma, and other biological fluids
Assay Type: Sandwich
Sensitivity: The minimum detectable dose of IL-8 was determined by adding two standard deviations to the mean optical density value of the 20 zero-standard replicates and calculating the corresponding concentration from the standard curve. The minimum detectable dose using a standard curve generated with Calibrator Diluent I is 10 pg/mL and using Calibrator Diluent II is 4 pg/mL.