IL-6 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; IL-6 elisa kit; IL-6;
The Human IL-6 il6 (Catalog #MBS355306) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS355306 ELISA Kit recognizes Human IL-6. MyBioSource\'s IL-6 can be used in a range of immunoassay formats including, but not limited to, Quantitative sELISA (EIA).For quantitative detection of IL-6 in human serum, plasma, body fluids, tissue lysates or cell culture supernates. Researchers should empirically determine the suitability of the IL-6 il6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The IL-6 il6 product has the following accession number(s) (GI #7549769) (NCBI Accession #NP_036721.1) (Uniprot Accession #P20607). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the IL-6 Human ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-6 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-6 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-6 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-6 can be calculated.
Background/Introduction: Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell surface signaling assembly composed of IL6, IL6RA, and the shared signaling receptor gp130. It acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 is one of the most important mediators of fever and of the acute phase response. It is capable of crossing the blood brain barrier and initiating synthesis of PGE2 in the hypothalamus, thereby changing the body\'s temperature setpoint. IL-6 is also essential for hybridoma growth and is found in many supplemental cloning media such as briclone. Inhibitors of IL-6 are used to treat postmenopausal osteoporosis. IL-6 is relevant to many diseases such as diabetes, atherosclerosis, depression, Alzheimer\'s Disease, systemic lupus erythematosus, prostate cancer, and rheumatoid arthritis.
Samples: Human serum, plasma, body fluids, tissue lysates or cell culture supernates
Assay Type: Quantitative Sandwich
Detection Range: 4.69 pg/ml-300 pg/ml
Sensitivity: < 0.3 pg/ml.