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IL-1beta elisa kit product blog

Posted on 2014-03-06 00:19:21 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; Interleukin 1beta; IL-1beta elisa kit; IL-1beta;
The Rat IL-1beta il1b (Catalog #MBS732184) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS732184 ELISA Kit recognizes Rat (Rattus, General) IL-1beta.

The IL-1beta il1b product has the following accession number(s) (GI #10835145) (NCBI Accession #NP_000567.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Interleukin 1beta ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) IL-1beta.

Principle of the assay: IL-1beta ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-IL-1beta antibody and an IL-1beta-HRP conjugate. The assay sample and buffer are incubated together with IL-1beta-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL-1beta concentration since IL-1beta from samples and IL-1beta-HRP conjugate compete for the anti-IL-1beta antibody binding site. Since the number of sites is limited, as more sites are occupied by IL-1beta from the sample, fewer sites are left to bind IL-1beta-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-1beta concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive or Sandwich
Detection Range: 50-1000pg/mL
Sensitivity: 1.0pg/ml. Intended Uses: This IL-1beta ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat IL-1beta. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Rat Interleukin 1beta ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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