Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow IL-1 elispot pair  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • CCL20 elisa kit blog
 • TG native protein blog
 • PLD5 elisa kit blog
 • RORA shrna blog
 • RACGAP1 sirna blog
 • FAM107B sirna blog
 • IgM secondary antibody blog
 • anti-CD105 antibody blog
 • CLC antibody pair blog
 • KIFC3 shrna blog
 • HDAC8 recombinant protein blog
 • BMP11 blocking peptide blog
 • TMEM158 shrna blog
 • [Met5, Lys6, Arg7]-Alpha-Neo-Endorphin blocking peptide blog
 • IL2RA recombinant protein blog
 • CRMP2 blocking peptide blog
 • CTSB inhibitor blog
 • ROS1 blocking peptide blog
 • NO elisa kit blog
 • STAT6 blocking peptide blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • BCAS2 Antibody
 • VAV2 (Phospho-Tyr142) Antibody
 • MKK3 (Ab-189) Antibody
 • CCT6A Antibody
 • POLR2A (Phospho-Ser1619) Antibody
 • DLC1 Antibody
 • Pro-Gastrin Releasing Peptide (PROGRP) ELISA Kit
 • Centriole and Centrosome Antibody (ACA) ELISA Kit
 • Inhibin B (INHB) ELISA Kit
 • Glycogen Phosphorylase Mm (GP-MM) ELISA Kit
 • Histone H2A-H2B-Dna Autoantibody (HHA) ELISA Kit
 • Nuclear Factor, Interleukin 3 Regulated (NFIL3) ELISA Kit
 • Remnant Lipoprotein - Cholesterol (RLP-C) ELISA Kit
 • Very Low Density Lipoprotein Receptor (VLDLR) ELISA Kit
 downarrow more ...
separator

IL-1 elispot pair product blog

separator
Posted on 2017-06-04 02:30:29 by mybiosource_staff
Share it on ...     
separator
separator
Tags: IL-1; ELISpot Pair; IL-1 elispot pair;
separator
The IL-1 n/a (Catalog #MBS335778) is an ELISpot Pair and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

IL-1 n/a

Assay Type: Sandwich.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions