Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow IL-1 elispot pair  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • PHA-665752 kinase inhibitor blog
 • PNLIP native protein blog
 • anti-IgA2 antibody blog
 • LUM elisa kit blog
 • RAB3C sirna blog
 • BQ-610 enzyme inhibitor blog
 • VEGF-B elisa kit blog
 • Trapidil inhibitor blog
 • alpha1-Acid glycoprotein (alpha1-AGP) elisa kit blog
 • anti-SCGB2A2 antibody blog
 • TIMD4 shrna blog
 • anti-MLXIPL antibody blog
 • anti-GPI antibody blog
 • IgG secondary antibody blog
 • TMEM72 sirna blog
 • anti-protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 antibody blog
 • Beta-Amyloid peptide blog
 • anti-KRT7 antibody blog
 • Ulex europaeus lectin blog
 • MT-ND2 blocking peptide blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Melanocyte stimulating hormone (MSH) ELISA Kit
 • DISC1 Antibody
 • Albumin Antibody
 • SORBS1-ponsin Antibody
 • Janus Kinase 3 (JAK3) ELISA Kit
 • Plasminogen Activator Inhibitor (PAI) ELISA Kit
 • Insulin (INS) ELISA Kit
 • cluster of differentiation 146, CD146 ELISA Kit
 • Klotho (KL) Recombinant Protein
 • Rab5 Antibody
 • CNOT2 Antibody
 • EGFLAM Antibody
 downarrow more ...

IL-1 elispot pair product blog

Posted on 2017-06-04 02:30:29 by mybiosource_staff
Share it on ...     
Tags: IL-1; ELISpot Pair; IL-1 elispot pair;
The IL-1 n/a (Catalog #MBS335778) is an ELISpot Pair and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtitre plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

IL-1 n/a

Assay Type: Sandwich.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions