IgG2a negative/isotype control product blog
Tags: IgG2a; Negative/Isotype Control; IgG2a negative/isotype control; MOUSE IgG2a NEGATIVE CONTROL;
The IgG2a n/a (Catalog #MBS238185) is a Negative Control produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MOUSE IgG2a NEGATIVE CONTROL can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: *. Researchers should empirically determine the suitability of the IgG2a n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
MBS238172 is suitable for use as a negative control reagent for the measurement of non-specific binding of mouse monoclonal antibodies of isotype IgG2a to human tissue. This antibody may not be suitable for intracellular staining on some cell types. Clone MRC OX-34 recognises a rat cell surface marker, and therefore cannot be used as a negative control in this species. This product is routinely tested in flow cytometry on rat splenocytes to confirm antibody activity and on human whole blood to test for suitability as a negative control. Test results have shown that MBS238172 is also suitable for use as a negative control with bovine, ovine, porcine, equine, canine, lapine and guinea-pig tissues.
Reconstitution: Reconstitute with 1.0 ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Activated rat T-helper cells.
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the NS1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Negative Control.