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IgG secondary antibody product blog

Posted on 2016-10-03 07:53:31 by mybiosource_staff
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Tags: IgG; Secondary Antibody; IgG secondary antibody; Goat anti Rabbit IgG (H + L) (Fab'2) (Texas Red);
The IgG n/a (Catalog #MBS538867) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Rabbit IgG (H + L) (Fab\'2) (Texas Red) reacts with Rabbit IgG (H -L) and may cross-react with other species as described in the data sheet. MyBioSource\'s Goat anti Rabbit IgG (H + L) (Fab\'2) (Texas Red) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF).
ELISA: 1:10,000-1:50,000
FC: 1:500-1:2,500
IF: 1:1,000-1:5,000. Researchers should empirically determine the suitability of the IgG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given secondary antibody. We\'ve tested the Goat anti Rabbit IgG (H + L) (Fab\'2) (Texas Red) with the following immunoassay(s):
Western Blot (WB) (Western blot of Texas Red conjugated Goat F(ab\')2 Anti-Rabbit IgG. Lane 1: Rabbit IgG. Lane 2: None. Load: 50 ng per lane. None. Texas Red Goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Rabbit IgG.)
Western Blot (WB) IgG.

Goat anti Rabbit IgG (H + L) secondary antibody (Fab\'2) (Texas Red).

Biological Significance: Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains - two heavy chains and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. The Fab region, (fragment, antigen binding), is the binding region of the antibody. Biohazard Information: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Tag/Conjugate: Texas Red
Immunogen: Goat anti-rabbit IgG (H + L) (Fab\'2) (Texas Red) was raised in goat using rabbit IgG whole molecule as the immunogen.
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