IgG secondary antibody product blog
Tags: IgG; Secondary Antibody; IgG secondary antibody; Rabbit anti Human IgG (H + L) (biotin);
The IgG n/a (Catalog #MBS538529) is a Secondary Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti Human IgG (H + L) (biotin) reacts with Human IgG (H - L) and may cross-react with other species as described in the data sheet. MyBioSource\'s Rabbit anti Human IgG (H + L) (biotin) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB).ELISA: 1:20,000-1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000. Researchers should empirically determine the suitability of the IgG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given secondary antibody. We\'ve tested the Rabbit anti Human IgG (H + L) (biotin) with the following immunoassay(s):
Western Blot (WB) (Western Blot of Biotin Conjugated Human IgG antibody. Lane 1: Human IgG. Lane 2: None. Load: 50 ng per lane. Human IgG antibody biotin conjugated at 1:1,000 for 60 min at RT. Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: Blocking buffer for 30 min at RT. Size: 25 & 55 kDa, 25 & 55 kDa for Human IgG.)
Rabbit anti Human IgG (H + L) secondary antibody (biotin).
Tag/Conjugate: Biotin
Immunogen: Rabbit anti-human IgG (H+L) (biotin) was raised in rabbit using human IgG whole molecule as the immunogen.