IgG secondary antibody product blog
Tags: IgG; Secondary Antibody; IgG secondary antibody; IgG [H&L];
The IgG n/a (Catalog #MBS417948) is a Secondary Antibody produced from Donkey and is intended for research purposes only. The product is available for immediate purchase. The Peroxidase Conjugated Affinity Purified anti-Goat IgG [H&L] [Donkey] reacts with Goat and may cross-react with other species as described in the data sheet. MyBioSource\'s IgG [H&L] can be used in a range of immunoassay formats including, but not limited to, Immunoblotting, Western Blot (WB), Dot Blot, ELISA (EIA), Immunoperoxidase Electron Microscopy, Immunohistochemistry (IHC), Other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.This product has been assayed against 1.0 ug of Goat IgG in a standard capture ELISA using ABTS (2, 2\'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:200,000 of the reconstitution concentration is suggested for this product. Researchers should empirically determine the suitability of the IgG n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Goat IgG and Goat Serum. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit and Rat Serum Proteins.
Immunogen: Goat IgG whole molecule. Storage Buffer: 0.02 M Potassium Phosphate,0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA), IgG and Protease free.