|The Rabbit IgG n/a (Catalog #MBS704077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS704077 ELISA Kit recognizes Rabbit (General) IgG.
The IgG n/a product has the following accession number(s) (GI #185362) (NCBI Accession #AAA02914.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the rabbit Immunoglobulin G, IgG ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Introduction: Immunoglobulin G (IgG) are antibody molecules. Each IgG is composed of four peptide chains -- two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. Other Immunoglobulins may be described in terms of polymers with the IgG structure considered the monomer. IgG is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. IgG molecules are synthesized and secreted by plasma B cells. IgG antibodies are predominantly involved in the secondary immune response (the main antibody involved in primary response is IgM). The presence of specific IgG generally corresponds to maturation of the antibody response. IgG is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in utero. Along with IgA secreted in the breast milk, residual IgG absorbed through the placenta provides the neonate with humoral immunity before its own immune system develops. Colostrum contains a high percentage of IgG, especially in bovine colostrum. IgG can bind to many kinds of pathogens, for example viruses, bacteria, and fungi, and protects the body against them by agglutination and immobilization, complement activation (classical pathway), opsonization for phagocytosis and neutralization of their toxins. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity(ADCC).IgG is also associated with Type II and Type III Hypersensitivity.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with IgG. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for IgG, mix well and incubated. The more the amount of IgG in samples, the less HRP-antibody bound by pre-coated IgG. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of IgG in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma
Detection Range: 0.047 ug/ml-12 ug/ml