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IgG elisa kit product blog

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Posted on 2014-03-26 16:24:51 by mybiosource_staff
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Tags: ELISA Kit; Rat ELISA Kit; IgG elisa kit; IgG; Immunoglobulin G, IgG;
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The Rat IgG n/a (Catalog #MBS700411) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700411 ELISA Kit recognizes Rat (Rattus, General) IgG.

The IgG n/a product has the following accession number(s) (GI #185362) (NCBI Accession #AAA02914.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Immunoglobulin G, IgG ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) IgG.

Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with rat IgG. Standards and samples are pipetted into the wells with a Horseradish Peroxidase (HRP) conjugated antibody specific for rat IgG. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in opposite to the amount of rat IgG in samples. The color development is stopped and the intensity of the color is measured.

Samples: Serum, plasma
Assay Type: Competitive
Detection Range: 31.25 ng/ml -8000 ng/ml.
Sensitivity: The minimum detectable dose of rat IgG is typically less than 31.25 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest rat IgG concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Rat Immunoglobulin G, IgG ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. IgG also interacts with the following gene(s): FLNA. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene.
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