IGF2 elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; IGF-2; IGF2 elisa kit; IGF2;
The Mouse IGF2 igf2 (Catalog #MBS175976) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS175976 ELISA Kit recognizes Mouse IGF2.The IGF2 igf2 product has the following accession number(s) (GI #124256) (NCBI Accession #P09535.1) (Uniprot Accession #P09535). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse IGF-2 PicoKine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The Picokine� Mouse IGF2 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Mouse IGF2 with a 96-well strip plate that is pre-coated with antibody specific for IGF2. The detection antibody is a biotinylated antibody specific for IGF2. The capture antibody is monoclonal antibody from rat, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Mouse IGF2 with immunogen: Expression system for standard: E.coli; Immunogen sequence: A25-E91. The kit is analytically validated with ready to use reagents. To measure Mouse IGF2, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly proportional to Mouse IGF2 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells\' readings against the standard curve to determine the concentration of Mouse IGF2 in the sample.!!Background/Introduction: Insulin-like growth factor II is also known as somatomedin A. IGF-2 is a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is paternally expressed in the fetus and placenta. IGF-II is a mitogen for many cell types and an important modulator of muscle growth and differentiation. IGF-II gene is prevalently expressed during prenatal development and its gene activity is regulated by genomic imprinting, in that the allele inherited from the father is active and the allele inherited from the mother is inactive in most normal tissues. IGF-II appears to be induced by placental lactogen during prenatal development. It is a mediator of prolactin-induced alveologenesis; prolactin, IGF-2, and cyclin D1, all of which are overexpressed in breast cancers, are components of a developmental pathway in the mammary gland.