Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow IFN elispot pair  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • MDV antibody IgM elisa kit blog
 • ADCYAP1 elisa kit blog
 • IgG control blog
 • DPYSL 2 elisa kit blog
 • anti-LGALS1 antibody blog
 • Tissue, Total Protein Adult Normal, Brain, Vermis Cerebelli lysate blog
 • anti-wCD8a antibody blog
 • anti-CD4 antibody blog
 • LZTS3 sirna blog
 • ALG13 shrna blog
 • TRIB3 sirna blog
 • IgG secondary antibody blog
 • PROCR blocking peptide blog
 • anti-AR antibody blog
 • Sancycline inhibitor blog
 • ELISA NEPTUNE SAMPLE DILUENT accessory reagent blog
 • Tissue, Total Protein, Monkey lysate blog
 • anti-ORAI1 antibody blog
 • anti-b-Amyloid antibody blog
 • IgG2b, Murine Isotype Control isotype control blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • phospho-JNK1 Antibody
 • RNF7 cDNA Clone
 • Melanocyte Stimulating Hormone (MSH) ELISA Kit
 • Serine protease inhibitor A3K (Serpina3k) Recombinant Protein
 • Neurotrophin-3 (NT-3) Recombinant (NTF3) Recombinant Protein
 • PLCXD1 Antibody
 • antithrombin III antiserum (SERPINC1) Antibody
 • cyclin-dependent kinase 4 (CDK4) Recombinant Protein
 • heat shock 70kD protein 12B (HSPA12B) ELISA Kit
 • Glutathione Peroxidase (GSH-PX) ELISA Kit
 • 4E-BP1 Antibody
 • erythropoietin (EPO) ELISA Kit
 • LAG-3:Fc Recombinant Protein
 downarrow more ...

IFN elispot pair product blog

Posted on 2016-09-01 07:13:39 by mybiosource_staff
Share it on ...     
Tags: IFN; ELISpot Pair; IFN elispot pair;
The IFN n/a (Catalog #MBS335776) is an ELISpot Pair and is intended for research purposes only. The product is available for immediate purchase. The Human IFN gamma ELISpot Pair reacts with No cross reactivity with other human cytokines and may cross-react with other species as described in the data sheet.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Intended Uses: ELISpot is a highly specific immunoassay for the analysis of cytokine and other soluble molecule production and secretion from T-cells at a single cell level in conditions closely comparable to the in-vivo environment with minimal cell manipulation. This technique is designed to determine the frequency of cytokine producing cells under a given stimulation and the comparison of such frequency against a specific treatment or pathological state. The ELISpot assay constitutes an ideal tool in the investigation of Th1 / Th2 responses, vaccine development, viral infection monitoring and treatment, cancerology, infectious disease, autoimmune diseases and transplantation. Utilising sandwich immuno-enzyme technology, ELISpot assays can detect both secreted cytokines and single cells that simultaneously produce multiple cytokines. Cell secreted cytokines or soluble molecules are captured by coated antibodies avoiding diffusion in supernatant, protease degradation or binding on soluble membrane receptors. After cell removal, the captured cytokines are revealed by tracer antibodies and appropriate conjugates.

Principle of the Assay: A capture antibody highly specific for the analyte of interest is coated to the wells of a PVDF bottomed 96 well microtiter plate either during kit manufacture or in the laboratory. The plate is then blocked to minimise any non-antibody dependent unspecific binding and washed. Cell suspension and stimulant are added and the plate incubated allowing the specific antibodies to bind any analytes produced. Cells are then removed by washing prior to the addition of Biotinylated detection antibodies which bind to the previously captured analyte. Enzyme conjugated streptavidin is then added binding to the detection antibodies. Following incubation and washing substrate is then applied to the wells resulting in coloured spots which can be quantified using appropriate analysis software or manually using a microscope.

IFN n/a

Assay Type: Sandwich.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions