|The Human Hsp60 hsp60 (Catalog #MBS494554) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494554 ELISA Kit recognizes Human (General) Hsp60.
The Hsp60 hsp60 product has the following accession number(s) (GI #85542006) (NCBI Accession #ABC71128.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Hsp60 (Anti-Human IgG/IgA/IgM) ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Enzyme-linked immunosorbent assay (ELISA) for the determination of antibodies to human Hsp60 in serum samples from human origin. This assay allows for reproducible, accurate, and precise determination of IgG, IgA, and IgM antibodies (total) to human Hsp60 in serum. For research use only, not for use in diagnostic procedures.
Detection Range: 0 / 7.81 - 250 ng/mL
Sensitivity: 2.88 ng/mL. Standards: 7 standards, serially diluted from 1 concentrated standard.
Sample Volume: 100 uL of properly diluted (1:1000) unknown / determination.
Design: Enzyme-linked immunosorbent assay (ELISA) technique.
Assay Description: 2 hour incubation (RT) + 1 hour (RT) + 15 min. (RT) = 3 hours, 15 min. total incubation time. Hsp60 (Anti-Human IgG/IgA/IgM) ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.