hsCRP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; C-Reactive Protein; hsCRP elisa kit; hsCRP;
The Human hsCRP crp (Catalog #MBS494066) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS494066 ELISA Kit recognizes Human hsCRP.The hsCRP crp product has the following accession number(s) (GI #55770842) (NCBI Accession #NP_000558.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the C-Reactive Protein (hsCRP)� ELISA with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only) ()
Intended Uses: For the determination of C-Reactive Protem by enzyme immunoassay in human serum.
Principle of the Assay: The principle of the following enzyme immunoassay test follows a typical two-step capture or \'sandwich\' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and calibrators are allowed to bind to the plate, washed, and subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The enzymatic reaction is tenninated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample A set of calibrators is used to plot a standard curve from which the amount of CRP in samples and controls can be directly read.
Samples: Human serum
Assay Type: Sandwich. Intra-assay Precision: Three samples were assayed ten times each on the same calibrator curve.
Inter-assay Precision: Three samples were assayed ten times over a period of four weeks. C-Reactive Protein (hsCRP)� ELISA or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Arthritis, Rheumatoid, Atherosclerosis, Cardiovascular Diseases, Heart Diseases, Infection, Inflammation, Metabolic Diseases, Myocardial Ischemia, Necrosis, Pain are some of the diseases may be linked to C-Reactive Protein (hsCRP)� ELISA. hsCRP also interacts with the following gene(s): CFH, FCGR2A, FN1, ICAM1, IL6, LEP, SERPINE1, STAT3. The following patways have been known to be associated with this gene.