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HPF4 elisa kit product blog

Posted on 2014-04-03 20:07:27 by mybiosource_staff
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Tags: ELISA Kit; Human ELISA Kit; HPF4; HPF4 elisa kit;
The Human HPF4 n/a (Catalog #MBS2515736) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2515736 ELISA Kit recognizes Human (General) HPF4.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human HPF4(Heparin/Platelet Factor 4 Antibodies)ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) HPF4.

Principle of the assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to HPF4. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HPF4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HPF4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm 2 nm. The OD value is proportional to the concentration of HPF4. You can calculate the concentration of HPF4 in the samples by comparing the OD of the samples to the standard curve.

Samples: Serum, Plasma, Biological Fluids
Assay Type: Sandwich
Detection Range: 0.625-40ng/mL
Sensitivity: Min: 0.375ng/mL; Max: 40ng/mL. Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human HPF4 concentrations in serum, plasma and other biological fluids. Human HPF4(Heparin/Platelet Factor 4 Antibodies)ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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