HP elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; HP elisa kit; Hydroxylysyl Pyridinoline;
The Human HP n/a (Catalog #MBS751881) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS751881 ELISA Kit recognizes Human HP.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human Hydroxylysyl Pyridinoline ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This Hy-PYD ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Hy-PYD. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: Hy-PYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-Hy-PYD antibody and an Hy-PYD-HRP conjugate. The assay sample and buffer are incubated together with Hy-PYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Hy-PYD concentration since Hy-PYD from samples and Hy-PYD-HRP conjugate compete for the anti-Hy-PYD antibody binding site. Since the number of sites is limited, as more sites are occupied by Hy-PYD from the sample, fewer sites are left to bind Hy-PYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Hy-PYD concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Quantitative Competitive
Sensitivity: 1.0 ng/mL. Human Hydroxylysyl Pyridinoline ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.