|The Human HNP1-3 n/a (Catalog #MBS701018) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS701018 ELISA Kit recognizes Human (General) HNP1-3.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human neutrophil peptide 1-3, HNP1-3 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HNP1-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HNP1-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HNP1-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HNP1-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Sandwich
Detection Range: 1.56 ng/ml-100 ng/ml
Sensitivity: 0.39ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human neutrophil peptide 1-3, HNP1-3 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.