|The Human HMW Adiponectin n/a (Catalog #MBS702778) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702778 ELISA Kit recognizes Human (General) HMW Adiponectin.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual
for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human High Molecular Weight Adiponectin, HMW Adiponectin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HMW Adiponectin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for HMW Adiponectin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3\',5,5\' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain HMW Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of HMW Adiponectin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range: 3.12ng/ml-200 ng/ml
Sensitivity: 0.78 ng/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul. Human High Molecular Weight Adiponectin, HMW Adiponectin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.