Gstm1 recombinant protein product blog
Tags: Recombinant Protein; GSTM1; GSTM1 recombinant protein; Glutathione S-Transferase Mu1;
The Gstm1 gstm1 (Catalog #MBS203525) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Glutathione S-Transferase Mu1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Gstm1 gstm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The Gstm1 gstm1 product has the following accession number(s) (GI #6754084) (NCBI Accession #NP_034488) (Uniprot Accession #P10649). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MGSSHHHHHH SSGLVPRGSH MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the Glutathione S-Transferase Mu1, 1-218aa, Mouse, His tag, E Coli (Bioactivity Validated) with the following immunoassay(s):
SDS-Page
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Glutathione S-Transferase Mu1 are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Antigen Species: Mouse
Tag Information: His-tag. Biological Activity: Specific activity is > 40,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol.
2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant GSTM1 protein 2.5ug/ml in assay buffer.
4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes. Bladder, Blood, Brain, Heart, Kidney, Liver, Lung, Prostate, Skin, Stomach tissues are correlated with this protein. Gstm1 also interacts with the following gene(s): Cyp1a1, Cyp1a2, Cyp1b1, Cyp2e1, Ephx1, Gsta3, Gstm2, Gstm3, Gstt1, Mgst1. Carcinoma, Squamous Cell, Chromosome Aberrations, Colorectal Neoplasms, Disease Susceptibility, Genetic Predisposition to Disease, Leukemia, Liver Diseases, Lung Neoplasms, Skin Diseases, Urinary Bladder Neoplasms are some of the diseases may be linked to Glutathione S-Transferase Mu1, 1-218aa, Mouse, His tag, E Coli (Bioactivity Validated). The following patways have been known to be associated with this gene.