GST recombinant protein product blog
Tags: GST; Recombinant Protein; Glutathione S-Transferase; GST recombinant protein;
The GST gst 26 (Catalog #MBS203158) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Glutathione S-Transferase (GST) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the GST gst 26 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The GST gst 26 product has the following accession number(s) (GI #160927) (NCBI Accession #AAB59203.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given active protein. We\'ve tested the Glutathione S-Transferase (GST), 1-218 aa, Schistosoma japonicum E Coli (Bioactivity Validated) with the following immunoassay(s):
SDS-Page
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Glutathione S-Transferase (GST) are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Antigen Species: Schistosoma japonicum
Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product. Biological Activity: Specific activity is > 20 units/mg, and is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C.
Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol.
2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer.
3. Add 20ul of recombinant GST protein 20ug/ml in assay buffer.
4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.