GSK3 positive control product blog
Tags: GSK3 BETA; GSK3; Positive Control; GSK3 positive control;
The GSK3 gsk3b (Catalog #MBS542832) is a Positive Control and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s GSK3 beta can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western Blot: 1:500. Researchers should empirically determine the suitability of the GSK3 gsk3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The GSK3 gsk3b product has the following accession number(s) (GI #20455502) (NCBI Accession #P49841.2) (Uniprot Accession #P49841). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Western Blot Positive Control Glycogen Synthase Kinase-3 beta
Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates. In skeletal muscle, contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. May also mediate the development of insulin resistance by regulating activation of transcription factors. Regulates protein synthesis by controlling the activity of initiation factor 2B (EIF2BE/EIF2B5) in the same manner as glycogen synthase. In Wnt signaling, GSK3B forms a multimeric complex with APC, AXIN1 and CTNNB1/beta-catenin and phosphorylates the N-terminus of CTNNB1 leading to its degradation mediated by ubiquitin/proteasomes. Phosphorylates JUN at sites proximal to its DNA-binding domain, thereby reducing its affinity for DNA.
Immunogen: Purified Protein
Immunoglobulin: IgY
Expression: Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain. Molecular Function: Developmental protein, Kinase, Serine/threonine-protein kinase, Signal tranduction inhibitor, Transferase
Structure: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
Contains 1 protein kinase domain.
Subcellular Location: Cytoplasm; Nucleus; Cell membrane. Alzheimer Disease, Bipolar Disorder, Brain Diseases, Cardiovascular Diseases, Depressive Disorder, Disease Models, Animal, Inflammation, Movement Disorders, Nervous System Diseases, Schizophrenia are some of the diseases may be linked to GSK3 beta Positive Control. GSK3 also interacts with the following gene(s): AKT1, APC, AXIN1, AXIN2, CTNNB1, FRAT1, JUN, LRP6, MAPT, MYC. Blood, Bone, Brain, Embryonic Tissue, Heart, Liver, Lung, Muscle, Ovary, Prostate tissues are correlated with this protein.