Goat Ig anti Human IgA (Fc specific) immunofixation secondary antibody product blog
Tags: Secondary Antibody; Goat Ig anti Human IgA (Fc specific) immunofixation; Goat Ig anti Human IgA (Fc specific) immunofixation secondary antibody;
The Goat Ig anti Human IgA (Fc specific) immunofixation n/a (Catalog #MBS571716) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat Ig anti Human IgA (Fc specific) immunofixation reacts with Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations o and may cross-react with other species as described in the data sheet. MyBioSource\'s Goat Ig anti Human IgA (Fc specific) immunofixation can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Researchers should empirically determine the suitability of the Goat Ig anti Human IgA (Fc specific) immunofixation n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
This antiserum is intended to detect and identify IgA in serum or other body fluids using the immunofixation technique. Like immunoelectrophoresis immunofixation is essentially a two step technique. Proteins in a complex mixture are separated by electrophoresis in a gel carrier, followed by immunoprecipitation in situ with the antiserum. Non-precipitated proteins are removed by washing and the precipitated complex is revealed with a protein stain which allows its exact localization. Immunofixation may be the method of choice whenever a high level of sensitivity is required to identify a minor protein component against a high background of other proteins. It enables the detection and identification of more than one paraproteins in serum or of free light chain. The detection limit is approximately 0.5 to 1 mg/ml in the presence of normal levels of immunoglobulins. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Preservative: No preservative added, as it may interfere with the antibody activity.
Immunogen: Highly purified polyclonal IgA and monoclonal IgA2 isolated from human serum. Freund\'s complete adjuvant is used in the first step of the immunization procedure.
Physicochemical characteristic: IgG protein concentration 10 mg/ml. No foreign proteins added.
Antibody titre: Precipitin titre not less than 1 :64 when tested against normal human serum in agar immunodiffusion block titration.
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibodies cross-reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum. Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to mammalian immunoglobulins, since homologous proteins of different species frequently share antigenic determinants. The degree of crossreactivity is also dependent on the concentRations of the reactants and the sensitivity of the assay arrangement. This antiSerum fraction has not been tested for cross-reactivity.
Caution: This product should be handled by qualified persons only and appropriate precautions should be taken in its handling and disposal, and of all associated materials. For in vitro laboratory research purposes only.