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Goat anti Rat IgE (Fc specific) secondary antibody product blog

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Posted on 2016-10-15 21:48:12 by mybiosource_staff
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Tags: Secondary Antibody; Goat anti Rat IgE (Fc specific); Goat anti Rat IgE (Fc specific) secondary antibody;
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The Goat anti Rat IgE (Fc specific) n/a (Catalog #MBS571837) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Rat IgE (Fc specific) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Goat anti Rat IgE (Fc specific) can be used in a range of immunoassay formats including, but not limited to, Indirect Immunofluorescence (IF), ELISA (EIA), Dot Blot (DB), Immunoblotting (IB).
When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established. Working dilutions for histochemical and cytochemical use are usually between 1:100 and 1:250; in ELISA and comparable non-preCipitating antibody-binding assays between 1 :500 and 1 :5,000. Researchers should empirically determine the suitability of the Goat anti Rat IgE (Fc specific) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


The reactivity of the antiserum is directed to the Fc subunits of the IgE molecule which represents strict isotype (class) specificity. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of IgE, a single precipitin line is obtained which shows a reaction of full identity with the precipitin line obtained with the used immunogen. It does not react with rat IgG, including all subclasses, IgA, IgM and IgG/Fab or any non-Ig protein in rat serum. As unlabelled secondary antibody for indirect detection of IgE in rat cell, tissue substrates and body fluids in immunofluorescence and immunoenzyme assay methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detecting antibody reagent in non-isotopic assay methodology (e.g. ELISA) to identify and measure IgE in rat serum or other body fluid. When applied in any immunocytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established.

Goat anti Rat IgE (Fc specific) n/a

Source: Purified homogenous IgE isolated from rat serum. Freund\'s complete adjuvant is used in the first step of the immunization procedure.
Product Type: Secondary Antibodies
Source: Purified homogenous IgE isolated from rat serum. Freundís complete adjuvant is used in the first step of the immunization procedure
Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required to eliminate antibody activity reacting with other components of the immunoglobulin system and to any other serum protein. Special attention is given to the elimination of antibodies to the common Fab portion of immunoglobulins. The use of insolubilized adsorption antigens prevents the prescence of excess adsorbent protein or immune complexes in the antiserum.
Preservative: No preservative added, as it may interfere with the antibody activity.
Immunogen: Purified homogenous IgE isolated from rat serum. Freund\'s complete adjuvant is used in the first step of the immunization.)f1 procedure.
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