Goat anti Human J chain of dimeric IgA secondary antibody product blog
Tags: Secondary Antibody; Goat anti Human J chain of dimeric IgA; Goat anti Human J chain of dimeric IgA secondary antibody;
The Goat anti Human J chain of dimeric IgA n/a (Catalog #MBS571564) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human J chain of dimeric IgA reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Goat anti Human J chain of dimeric IgA can be used in a range of immunoassay formats including, but not limited to, Precipitation assays.Precipitation assays. In immunoelectrophoresis use 2 μl serum or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Goat anti Human J chain of dimeric IgA n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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The reactivity of the antiserum is restricted to J chain as tested in immunoelectrophoresis and radial immunodiffusion. The antiserum shows a single precipitation reaction with totally reduced and alkylated polyclonal and monoclonal polymeric IgA and IgM. No reaction is obtained with the intact polyclonal or monoclonal IgA, IgM and IgG. There is also no detectable reaction with normal human serum, normal human milk, purified Ig heavy and light chains and secretory component. However a single precipitin line can be obtained with human serum after two hours incubation with 9 M urea and 0.2 M mercaptoethanol at pH 8.6, and this shows a reaction of identity with the precipitin line obtained with the purified immunogen. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion to identify the presence of free J chain in human serum or other body fluids and to determine its concentration. Since the J chain is folded within the polymeric structure of the immunoglobulin its epitopes are exposed only to a very limited degree. Although the antiserum may react with epitopes of the intact polymeric immunoglobulin, this will be normally not lead to a visible precipitation. When used in agar gel immunodiffusion, special consideration should be given to the small molecular size of the J chain, which results in a rapid diffusion into the gel layer. To prevent the development of an antigen excess during the second step of the immunoelectrophoresis, the antiserum should be applied immediately after the electrophoresis of the test sample. In double radial immunodiffusion (Ouchterlony) the antiserum should be applied first, followed by free J chain or reference preperation. Normal human serum can be used as a negative control.
Source: Human J chain is a polypeptide folded within the polymeric structure of the immunoglobulin. J chain isolated form human polymeric IgA and IgM are identical by criteria of composition, peptide maps and antigenicity. Human J chain has been established as distinct from all other component chains of polymeric IgA and IgM. It has an unique primary structure. Antisera to light chains or heavy chains of human Immunoglobulins do not react with J chain. J chain is released from dimeric human myeloma IgA by sulphitolysis and purified by ion exchange chromatography in urea and gel filtration in guanidine. Its purity is tested in alkaline urea polyacrylamide gel electrophoresis, in immunodiffusion and immunoelectrophoresis, showing it to be free of IgA, heavy or light chain contaminants. Freund�s complete adjuvant is used in the first step of the immunization procedure.