Goat anti Human IgG (heavy and light chains) secondary antibody product blog
Tags: Secondary Antibody; Goat anti Human IgG (heavy and light chains); Goat anti Human IgG (heavy and light chains) secondary antibody;
The Goat anti Human IgG (heavy and light chains) n/a (Catalog #MBS571163) is a Secondary Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti Human IgG (heavy and light chains) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Goat anti Human IgG (heavy and light chains) can be used in a range of immunoassay formats including, but not limited to, Precipitation assays.Precipitation assays. In immunoelectrophoresis use 2 ul or equivalent against 120 ul antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 ul antiserum in a 3 mm diameter centre well and 2 ul serum samples (neat and diluted) in 2 mm diameter peripheral wells. Researchers should empirically determine the suitability of the Goat anti Human IgG (heavy and light chains) n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against human serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG; as secondary antibody to precipitate the immunoglobulin in normal human serum; to prepare an immunoadsorbent for the production of Ig-free human serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.
Source: Purified normal IgG isolated from pooled human serum. Freund�s complete adjuvant is used in the first step of the immunization procedure.
Product: Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. It is reconstituted by adding 1 ml sterile distilled water. Reactivity Note: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog + Horse +/- pigeon - Sheep - Cat + Guinea Pig + Monkey ++ Rabbit + Swine + Chicken - Hamster +/- Mouse +/- Rat + Turkey -.