gh recombinant protein product blog
Tags: Growth Hormone; Recombinant Protein; GH recombinant protein;
The growth hormone gh (Catalog #MBS400007) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase.The growth hormone gh product has the following accession number(s) (GI #9256241) (NCBI Accession #AAB19750.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first ten N-terminal amino acids of recombinan t Gilthead Seabream growth hormone was determined and was found to be Ala-Gln-Pr o-Ile-Thr- Asp-Gly-Gl n-Arg-Leu.
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Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including growth hormone are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized recombinant Gilthead Seabream growth hormone in sterile 0.4% NaHCO3 adjusted, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation of recombinant Gilthead Seabream growth hormone was carried out by UV spectroscopy at 280 nm using the absorbency value of 0.71 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the DNAman computer analysis program of protein sequences.
Dimers and Aggregates: The purified recombinant Gilthead Seabream growth hormone consists of > 97% monomers as determined by gel-filtration chromatography. Endotoxin: Less than 0.1 ng/ug (IEU/ug) of recombinant Gilthead Seabream growth hormone.
Formulation: The recombinant Gilthead Seabream growth hormone was lyophilized from a concentrated (1mg/ml) solution with 0.02% NaHCO3.
Biological Activity: Binding assays of the 125I-labeled recombinant Gilthead Seabream growth hormone to dolphin fish liver microsomal fraction resulted in high specific binding characterized by a Ka of 1.93 nM and a Bmax of 540 fmol/mg microsomal fraction protein. Recombinant Gilthead Seabream growth hormone like ovine placental lactogen, exhibited growth-stimulating activity when applied orally to S.aurata larvae or intraperitoneally to juvenile fish.