GAD65 elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; GAD65; Glutamic acid decarboxylase 65; GAD65 elisa kit;
The Human GAD65 gad2 (Catalog #MBS281731) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS281731 ELISA Kit recognizes Human GAD65.The GAD65 gad2 product has the following accession number(s) (GI #4503875) (NCBI Accession #NP_000809.1) (Uniprot Accession #Q05329). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Human Glutamic acid decarboxylase 65 (GAD65) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate GAD65 in Human serum, plasma. An antibody specific for GAD65 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GAD65 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAD65 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAD65 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Sandwich
Detection Range: 0.78 ng/mL - 50 ng/mL
Sensitivity: 0.39 ng/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<12%. Human Glutamic acid decarboxylase 65 (GAD65) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. Brain, Pancreas tissues are correlated with this protein. The following patways have been known to be associated with this gene. GAD65 also interacts with the following gene(s): ABAT, GAD1, GLUL, INS, SLC32A1. Brain Diseases, Depressive Disorder, Epilepsy, Inflammation, Mental Disorders, Movement Disorders, Nervous System Diseases, Schizophrenia, Seizures, Substance Withdrawal Syndrome are some of the diseases may be linked to Human Glutamic acid decarboxylase 65 (GAD65) ELISA Kit.