|The Rabbit FVIIIAg n/a (Catalog #MBS722433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722433 ELISA Kit recognizes Rabbit (General) FVIIIAg.
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for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Factor VIII-related Antigen ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Intended Uses: This F8Ag ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit F8Ag. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay: F8Ag ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F8Ag antibody and an F8Ag-HRP conjugate. The assay sample and buffer are incubated together with F8Ag-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F8Ag concentration since F8Ag from samples and F8Ag-HRP conjugate compete for the anti-F8Ag antibody binding site. Since the number of sites is limited, as more sites are occupied by F8Ag from the sample, fewer sites are left to bind F8Ag-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F8Ag concentration in each sample is interpolated from this standard curve.
Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Rabbit Factor VIII-related Antigen ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.