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FVIIIAg elisa kit product blog

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Posted on 2013-11-04 20:47:42 by mybiosource_staff
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Tags: ELISA Kit; Rabbit ELISA Kit; Factor VIII-Related Antigen; FVIIIAg elisa kit; FVIIIAg;
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The Rabbit FVIIIAg n/a (Catalog #MBS722433) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722433 ELISA Kit recognizes Rabbit (General) FVIIIAg.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Factor VIII-related Antigen ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve
Typical Testing Data/Standard Curve FVIIIAg.

Intended Uses: This F8Ag ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit F8Ag. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: F8Ag ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-F8Ag antibody and an F8Ag-HRP conjugate. The assay sample and buffer are incubated together with F8Ag-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the F8Ag concentration since F8Ag from samples and F8Ag-HRP conjugate compete for the anti-F8Ag antibody binding site. Since the number of sites is limited, as more sites are occupied by F8Ag from the sample, fewer sites are left to bind F8Ag-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The F8Ag concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Rabbit Factor VIII-related Antigen ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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