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Free-T3 elisa kit product blog

Posted on 2015-02-27 03:06:26 by mybiosource_staff
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Tags: ELISA Kit; Rabbit ELISA Kit; Free Tri-Iodothyronine; FREE-T3 elisa kit; FREE-T3;
The Rabbit Free-T3 n/a (Catalog #MBS722578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722578 ELISA Kit recognizes Rabbit (General) Free-T3.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Free Tri-iodothyronine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Free-T3.

Principle of the Assay: FREE-T3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FREE-T3 antibody and an FREE-T3-HRP conjugate. The assay sample and buffer are incubated together with FREE-T3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FREE-T3 concentration since FREE-T3 from samples and FREE-T3-HRP conjugate compete for the anti-FREE-T3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FREE-T3 from the sample, fewer sites are left to bind FREE-T3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FREE-T3 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Intended Uses: This FREE-T3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit FREE-T3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Rabbit Free Tri-iodothyronine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
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