Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow Free-T3 elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • IL1B2 elisa kit blog
 • RPS28 shrna blog
 • TMB accessory reagent blog
 • anti-OAT antibody blog
 • anti-ENTPD1 antibody blog
 • ENTPD2 shrna blog
 • PB19V elisa kit blog
 • mmu-let-7f-5p mirna blog
 • FAM189A1 sirna blog
 • anti-CA1 antibody blog
 • PDE4DIP sirna blog
 • ASH2L sirna blog
 • MUC5B elisa kit blog
 • PF 429242 inhibitor blog
 • S17AH elisa kit blog
 • IFN beta 1a recombinant protein blog
 • CAMTA2 sirna blog
 • GNL3 blocking peptide blog
 • AP12 elisa kit blog
 • DDX58 shrna blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Renin ELISA Kit
 • Rotavirus NCDV (all antigens) Antibody
 • GPX4 Antibody
 • 60S acidic ribosomal protein P1 (RPLP1) Antibody
 • p53 Antibody
 • GDF2 Antibody
 • Growth Hormone Receptor (GHR) Antibody
 • Leptin Recombinant Protein
 • TGFBR2 Antibody
 • O-acetylserine sulfhydrylase (cysK) Recombinant Protein
 • Glycogen phosphorylase BB, GP-BB ELISA Kit
 • Curcumin Antibody
 • Carbonic Anhydrase I (CA1) ELISA Kit
 • Dual oxidase 1 (DUOX1 ) Antibody
 downarrow more ...

Free-T3 elisa kit product blog

Posted on 2015-02-27 03:06:26 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rabbit ELISA Kit; Free Tri-Iodothyronine; FREE-T3 elisa kit; FREE-T3;
The Rabbit Free-T3 n/a (Catalog #MBS722578) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS722578 ELISA Kit recognizes Rabbit (General) Free-T3.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rabbit Free Tri-iodothyronine ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) Free-T3.

Principle of the Assay: FREE-T3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-FREE-T3 antibody and an FREE-T3-HRP conjugate. The assay sample and buffer are incubated together with FREE-T3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FREE-T3 concentration since FREE-T3 from samples and FREE-T3-HRP conjugate compete for the anti-FREE-T3 antibody binding site. Since the number of sites is limited, as more sites are occupied by FREE-T3 from the sample, fewer sites are left to bind FREE-T3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FREE-T3 concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Detection Range: 5.0-100pg/mL
Sensitivity: 1.0pg/mL. Intended Uses: This FREE-T3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit FREE-T3. This ELISA kit for research use only, not for therapeutic or diagnostic applications!. Rabbit Free Tri-iodothyronine ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions