FIX elisa kit product blog
Tags: ELISA Kit; Mouse ELISA Kit; FIX; FIX elisa kit;
The Mouse FIX f9 (Catalog #MBS2502581) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2502581 ELISA Kit recognizes Mouse FIX.The FIX f9 product has the following accession number(s) (GI #80479046) (NCBI Accession #AAI09215.1) (Uniprot Accession #P00740). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Mouse FIX(Coagulation Factor IX)ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Intended Uses: This ELISA kit applies to the in vitro quantitative determination of MouseC5a concentrations in serum, pl asma and other biological fluids.
Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has beenpre-coated with an antibody specific to MouseC5a. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for MouseC5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain MouseC5a, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of MouseC5a. You can calculate the concentration of MouseC5a in the samples by comparing the OD of the samples to the standard curve.
Samples: Serum, Pl Asma And Other Biological Fluids
Assay Type: Quantitative Sandwich
Detection Range: 62.5-4000pg/mL
Sensitivity: 37.5pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level MouseC5a were tested 20times on one plate, respectively
Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level MouseC5a were testedon 3 different plates, 20 replicates in eachplate. Mouse FIX(Coagulation Factor IX)ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.