Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow FE elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • ADC shrna blog
 • ETS2 blocking peptide blog
 • Anti MUSK elisa kit blog
 • SRPRB sirna blog
 • Amyloid Beta-Protein biologically active peptide blog
 • SEPT5 shrna blog
 • anti-TCR, alpha, beta antibody blog
 • 14-3-3 epsilon recombinant protein blog
 • Streptavidin secondary antibody blog
 • HSV-2 antigen blog
 • C1INH elisa kit blog
 • mIL 1 beta recombinant protein blog
 • EMX1 shrna blog
 • anti-TLR9 antibody blog
 • GFAP elisa kit blog
 • MAP10 sirna blog
 • BEAN1 sirna blog
 • Adrenomedullin (13-52) peptide blog
 • KAT6B sirna blog
 • anti-GPR114 antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • AOX1 Antibody
 • Vaspin (SERPINA12) Recombinant Protein
 • Activation-induced Cytidine Deaminase (AICDA) Antibody
 • Triclocarban-13C6 Biochemical
 • CHD7 Antibody
 • Glutamate [NMDA] receptor subunit epsilon-1, GRIN2A ELISA Kit
 • Interleukin 12, IL-12-70 ELISA Kit
 • Protein delta homolog 1 (DLK1) Antibody
 • Interleukin 1beta (IL-1beta) ELISA Kit
 • Angiotensinogen (AGT) ELISA Kit
 • MDR3 (ABCB4) Antibody
 • TRABID-ZRANB1 Antibody
 • CD28 Antibody
 • IgG4 (Immunoglobulin G4) CLIA Kit
 downarrow more ...
separator

FE elisa kit product blog

separator
Posted on 2014-01-02 05:06:12 by mybiosource_staff
Share it on ...     
separator
separator
Tags: ELISA Kit; Porcine ELISA Kit; FE elisa kit; Ferritin (FE);
separator
The Porcine FE n/a (Catalog #MBS2019023) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS2019023 ELISA Kit recognizes Porcine (Pig, Swine, General) FE.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Ferritin (FE) ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) FE.

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ferritin (FE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ferritin (FE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ferritin (FE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ferritin (FE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Type: Double-antibody Sandwich
Samples: Serum, Plasma, Tissue homogenates and Other Biological Fluids
Detection Range: 0.94-60ng/mL
Sensitivity: < 0.42ng/mL. Application: Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ferritin (FE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ferritin (FE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =: SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%. Ferritin (FE) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions