F-actin elisa kit product blog
Tags: ELISA Kit; Human ELISA Kit; F-actin; F-actin elisa kit;
The Human F-actin n/a (Catalog #MBS702018) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS702018 ELISA Kit recognizes Human F-actin.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Human F-actin ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for F-actin has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any F-actin present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for F-actin is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of F-actin bound in the initial step. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma, tissue homogenates
Assay Type: Quantitative Sandwich
Detection Range: 0.625 ng/ml-40 ng/ml
Sensitivity: < 0.156 ng/ml. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess. Human F-actin ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.