Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow esRAGE elisa kit  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • APOBEC1 peptide blog
 • CYTH1 sirna blog
 • LEP recombinant protein blog
 • PRMT8 elisa kit blog
 • anti-Escherichia coli, K99 Pilus antibody blog
 • ORC6L peptide blog
 • anti-PCDH1 antibody blog
 • STPG2 shrna blog
 • Tissue, Section Tumor, Pelvic Tumor, Malignant Mesothelioma tissue section blog
 • AVEN blocking peptide blog
 • FNTB sirna blog
 • anti-DPYD antibody blog
 • FRS2 blocking peptide blog
 • anti-CD43 antibody blog
 • PRSS54 sirna blog
 • anti-ITGAM antibody blog
 • anti-BGLAP antibody blog
 • NT-proBNP elisa kit blog
 • IQCA1P1 sirna blog
 • anti-TG antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Chorionic Gonadotropin (CG) ELISA Kit
 • Peptide deformylase  Recombinant Protein
 • Actin, Muscle Speci?c (LOC100533357) Antibody
 • IL17A Antibody
 • carbonic anhydrase IX (CA9) ELISA Kit
 • IMPDH2 Antibody
 • SLC10A1 Antibody
 • cysteinyl-tRNA synthetase (CARS) ELISA Kit
 • Prelamin-A (LMNA) Recombinant Protein
 • Bradykinin (BK) ELISA Kit
 • Boc-Gln-Arg-Arg-MCA Enzyme Substrate
 • PRKCB Antibody
 • CRP (C-Reactive Protein) Antibody
 • CLCN7 Antibody
 downarrow more ...

esRAGE elisa kit product blog

Posted on 2014-10-09 19:42:12 by mybiosource_staff
Share it on ...     
Tags: ELISA Kit; Rat ELISA Kit; Endogenous Secretory Receptor For Advanced Glycation End Products; ESRAGE elisa kit; ESRAGE;
The Rat esRAGE ager (Catalog #MBS749652) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS749652 ELISA Kit recognizes Rat (Rattus, General) esRAGE.

The esRAGE ager product has the following accession number(s) (GI #84570471) (NCBI Accession #BAE72665.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

(Or you can also download the PDF Manual for complete product instructions).

Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Rat Endogenous secretory receptor for Advanced Glycation end Products ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only) esRAGE.

Intended Uses: This esRAGE ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat esRAGE. This ELISA kit for research use only, not for therapeutic or diagnostic applications!

Principle of the Assay: esRAGE ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-esRAGE antibody and an esRAGE-HRP conjugate. The assay sample and buffer are incubated together with esRAGE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the esRAGE concentration since esRAGE from samples and esRAGE-HRP conjugate compete for the anti-esRAGE antibody binding site. Since the number of sites is limited, as more sites are occupied by esRAGE from the sample, fewer sites are left to bind esRAGE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The esRAGE concentration in each sample is interpolated from this standard curve.

Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type: Competitive
Sensitivity: 0.1 ng/mL. Rat Endogenous secretory receptor for Advanced Glycation end Products ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. esRAGE also interacts with the following gene(s): Nfkb1, S100b.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions