|The ENPP2 enpp2 (Catalog #MBS542552) is a Positive Control and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s ENPP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
Western Blot: 1:250. Researchers should empirically determine the suitability of the ENPP2 enpp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ENPP2 enpp2 product has the following accession number(s) (GI #119612394) (NCBI Accession #EAW91988.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Western Blot Positive Control ectonucleotide pyrophosphatase/phosphodiesterase 2 (Autotaxin) C-epitope
Hydrolyzes lysophospholipids to produce lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Also can act on sphingosylphosphphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development. Tumor cell motility-stimulating factor.
Immunogen: Purified Protein
Expression: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues. Determinant: C-epitope
Molecular Function: alkylglycerophosphoethanolamine phosphodiesterase activity; calcium ion binding; hydrolase activity; lysophospholipase activity; nucleic acid binding; nucleotide diphosphatase activity; phosphodiesterase I activity; polysaccharide binding; scavenger receptor activity; transcription factor binding; zinc ion binding
Structure: Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Contains 2 SMB (somatomedin-B) domains.
Subcellular Location: Secreted. The following patways have been known to be associated with this gene. Bone, Brain, Embryonic Tissue, Heart, Liver, Lung, Pituitary Gland, Skin, Thyroid, Vascular tissues are correlated with this protein. Breast Neoplasms, Cardiovascular Diseases, Disease Models, Animal, Fibrosis, Hyperplasia, Inflammation, Liver Diseases, Lung Neoplasms, Necrosis, Neoplasm Metastasis are some of the diseases may be linked to ENPP2 Positive Control.