|The EheI n/a (Catalog #MBS638489) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.
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5\'-G G C^G C C-3\'.
Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Digestion of Agarose-embedded DNA: A minimum of 20 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: EheI does not cut GGm5CGCC. Blocked by CG methylation.
pACYC184: 4. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with EheI, more than 80% of the pBR322 DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5\'-termini concentration of 0.35µM. More than 90% of these can be recut.
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with EheI (see Star Activity).
Stability During Prolonged Incubation: A minimum of 1.0 unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-PstI fragments in 1 hour at 37 degree C in 50ul of assay buffer.