Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Blog  rightarrow EheI restriction enzyme  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • Single subunit botrocetin recombinant protein blog
 • ZIC1 blocking peptide blog
 • IFN lambda 2 recombinant protein blog
 • RASSF2 blocking peptide blog
 • CD5/Lyt-1 secondary antibody blog
 • GRIP1 sirna blog
 • anti-G10 antibody blog
 • anti-TRIM55 antibody blog
 • anti-IL-6 antibody blog
 • HSPG elisa kit blog
 • anti-NTRK2 antibody blog
 • RBBP7 sirna blog
 • MOR-BSA2 antigen blog
 • anti-EDN1 antibody blog
 • C1QL2 elisa kit blog
 • Substance P Serum peptide antisera blog
 • KIZ sirna blog
 • SNPH recombinant protein blog
 • FPR1 blocking peptide blog
 • PPP1R13B sirna blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • Defensin Alpha 5, Paneth Cell Specific (DEFalpha5) ELISA Kit
 • V5-TAG Antibody
 • HS3ST1 Antibody
 • HIST2H2BE cDNA Clone
 • Glutamate Transporter EAAC1 (SLC1A1) Antibody
 • GDP-fucose protein O-fucosyltransferase 1 (POFUT1) Recombinant Protein
 • ANXA9 Antibody
 • Cholic acid Antibody
 • S-2-Acetylthio-3-phenylprop ionic Acid Biochemical
 • Protein Tyrosine Kinase (PTK) ELISA Kit
 • Osteopontin (Spp1) Antibody
 • Aspartate carbamoyltransferase (pyrB) Recombinant Protein
 • WD repeat-containing protein CNBH2930 (CNBH2930), partial Recombinant Protein
 • [Protein-PII] uridylyltransferase (glnD), partial Recombinant Protein
 downarrow more ...
separator

EheI restriction enzyme product blog

separator
Posted on 2015-08-30 19:18:39 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Restriction Enzyme; EheI; EheI restriction enzyme;
separator
The EheI n/a (Catalog #MBS638489) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


5\'-G G C^G C C-3\'.

EheI n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Digestion of Agarose-embedded DNA: A minimum of 20 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: EheI does not cut GGm5CGCC. Blocked by CG methylation.
pACYC177: 0
pACYC184: 4. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda: 1
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with EheI, more than 80% of the pBR322 DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5\'-termini concentration of 0.35µM. More than 90% of these can be recut.
M13mp18/19: 1
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with EheI (see Star Activity).
pBR322: 4
PhiX174: 2
pTZ19R/U: 0
pUC18/19: 1
pUC57: 1
Stability During Prolonged Incubation: A minimum of 1.0 unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-PstI fragments in 1 hour at 37 degree C in 50ul of assay buffer.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions