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EheI restriction enzyme product blog

Posted on 2015-08-30 19:18:39 by mybiosource_staff
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Tags: Restriction Enzyme; EheI; EheI restriction enzyme;
The EheI n/a (Catalog #MBS638489) is a Restriction Enzyme and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

5\'-G G C^G C C-3\'.

EheI n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Digestion of Agarose-embedded DNA: A minimum of 20 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Methylation Effects: EheI does not cut GGm5CGCC. Blocked by CG methylation.
pACYC177: 0
pACYC184: 4. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours.
Lambda: 1
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with EheI, more than 80% of the pBR322 DNA fragments can be ligated in a reaction mixture containing 20-40u of T4 DNA ligase/1ug of fragments and 10% PEG at a 5\'-termini concentration of 0.35µM. More than 90% of these can be recut.
M13mp18/19: 1
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with EheI (see Star Activity).
pBR322: 4
PhiX174: 2
pTZ19R/U: 0
pUC18/19: 1
pUC57: 1
Stability During Prolonged Incubation: A minimum of 1.0 unit of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA-PstI fragments in 1 hour at 37 degree C in 50ul of assay buffer.
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