EGFR peptide product blog
Tags: EGFR; Peptide; Epidermal Growth Factor Receptor; EGFR peptide;
The EGFR egfr (Catalog #MBS658816) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Epidermal Growth Factor Receptor can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Researchers should empirically determine the suitability of the EGFR egfr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The EGFR egfr product has the following accession number(s) (GI #326467049) (NCBI Accession #ADZ75461.1) (Uniprot Accession #P00533). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given peptide. We\'ve tested the Epidermal Growth Factor Receptor, phosphorylated (Tyr1068) Blocking Peptide (erbB, EGFR), with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of EGF stimulated and unstimulated A431 cell lysates, using Phospho-EGF Receptor (Tyr1068) Antibody (left) and the same antibody pretreated with specific phospho-peptide (right).)
Epidermal growth factor (EGF) receptor is a 170kD single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation (1,2). Tyrosine 845 resides in the activation loop of the receptor kinase domain (3,4). Phosphorylation of Tyr845 may stabilize the activation loop, maintain the enzyme in an active state and provide a binding surface for substrate proteins. It has been shown that c-Src is involved in phosphorylation of the EGF receptor on Tyr845, which is associated with modulation of receptor function (5). Phospho-tyrosine 992 of activated EGF receptor is a direct binding site for the phospholipase C-gamma (PLC-gamma) SH2 domain (6). This binding results in activation of PLC-gamma-mediated downstream signaling. Phosphorylation of Tyr1045 creates a major docking site for c-Cbl (7). Binding of c-Cbl to the activated EGF receptor leads to assembly of ubiquitination machinery to the receptor, and enables receptor ubiquitination and degradation (8). Phospho-tyrosine 1068 of activated EGF receptor is a direct binding site for the Grb2/SH2 domain (9). This binding results in Ras activation through a Grb2/Sos-1 signaling mechanism (2)
Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Tyr1068 of human EGF receptor.
The following patways have been known to be associated with this gene. Adenocarcinoma, Breast Neoplasms, Carcinoma, Carcinoma, Non-Small-Cell Lung, Carcinoma, Squamous Cell, Disease Progression, Kidney Diseases, Lung Neoplasms, Neoplasms, Small Cell Lung Carcinoma are some of the diseases may be linked to Epidermal Growth Factor Receptor, phosphorylated (Tyr1068) Blocking Peptide (erbB, EGFR),.