EGF recombinant protein product blog
Tags: EGF; Recombinant Protein; Epidermal Growth Factor; EGF recombinant protein;
The EGF egf (Catalog #MBS142209) is an Active Protein produced from Pichia Pastoris and is intended for research purposes only. The product is available for immediate purchase.The EGF egf product has the following accession number(s) (GI #74749288) (NCBI Accession #Q6QBS2) (Uniprot Accession #Q6QBS2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Ser-As p-Ser-Glu, which agrees with the sequence of native EGF human.N-te rminal methionine has been completely removed enzymatica lly.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Our knowledge of the role of proteins in cellular processes is continually evolving. Most proteins, including Epidermal Growth Factor are typically involved in one or more signaling pathways or biological processes. Professionally manufactured recombinant proteins are increasingly becoming essential and commonplace tools for elucidating new knowledge about the role of proteins in both health and disease.
Solubility: It is recommended to reconstitute the lyophilized Epidermal Growth Factor in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Protein Content: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 2.858 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard.
Biological Activity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is < 0.1 ng/ml corresponding to a specific activity of 10,000,000Units/mg. Brain, Embryonic Tissue, Eye, Intestine, Kidney, Lung, Mouth, Muscle, Placenta, Skin tissues are correlated with this protein.