E2 elisa kit product blog
Tags: ELISA Kit; Canine ELISA Kit; Estradiol, E2; E2 elisa kit;
The Canine E2 n/a (Catalog #MBS700358) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS700358 ELISA Kit recognizes Canine E2.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given elisa kit. We\'ve tested the Canine Estradiol, E2 ELISA Kit with the following immunoassay(s):
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2, then incubated. Then substrate solutions are added to the wells, respectively. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of E2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samples: Serum, plasma, tissue homogenates
Assay Type: Competitive
Detection Range: 25 pg/ml-1000 pg/ml
Sensitivity: 25 pg/ml. Detection Wavelength: 450 nm
Sample Volume: 50-100ul
Protein Biological Process 1: Sex hormone. Canine Estradiol, E2 ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.