E2 elisa kit product blog
Tags: ELISA Kit; Fish ELISA Kit; E2 elisa kit; ESTRADIOL;
The Fish E2 n/a (Catalog #MBS283228) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS283228 ELISA Kit recognizes Fish E2.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Intended Uses: For the quantitative detection of Fish Estradiol (E2) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for E2 and Horseradish Peroxidase (HRP) conjugated E2. The competitive inhibition reaction is launched between with HRP labeled E2 and unlabeled E2 with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of E2 in the sample. The color development is stopped and the intensity of the color is measured.
Samples: Serum, plasma and other biological fluids
Assay Type: Quantitative Competitive
Detection Range: 40 pg/mL - 1000 pg/mL
Sensitivity: 20 pg/mL. Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV(%) = SD/meanX100. Inter-Assay: CV<10%. Fish Estradiol (E2) ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit.