Dulbecco's MEM culture media product blog
Tags: Culture Media; Dulbecco's MEM; Dulbecco's MEM culture media;
The Dulbecco\'s MEM n/a (Catalog #MBS653056) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Dulbecco\'s MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Endotoxin Level: < 1 EU/ml
pH: 7.0
Solubility: Pink, clear, complete
Directions per Liter: Dissolve 12.048g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not auto clave. Contains heat-labile compounds that can be damaged with auto claving. Components shown as g/liter: Inorganic Salts:
Calcium Chloride 2H2O: 0.154
Cupric Sulfate�H2O: 0.0000013
Ferric Nitrate�9H2O: 0.00005
Ferrous Sulfate�7H2O: 0.000417
Magnesium Chloride 6H2O: 0.0612
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.3118
Sodium Chloride: 6.996
Sodium Phosphate dibasic: 0.07102
Sodium Phosphate monobasic: 0.0543
Zinc Sulfate�7H2O: 0.000432
Amino Acids:
L-Alanine: 0.00445
L-Arginine�HCl: 0.1475
L-Asparagine�H2O: 0.0075
L-Aspartic Acid: 0.00665
L-Cystine�2HCl: 0.03129
L-Cysteine�HCl�H2O: 0.01756
L-Glutamic Acid : 0.00735
L-Glutamine: 0.365
Glycine: 0.01875
L-Histidine HCl�H2O: 0.03148
L-Isoleucine: 0.05447
L-Leucine: 0.05905L
L-Lysine�HCl: 0.09125
L-Methionine: 0.01724
L-Phenylalanine: 0.03548
L-Proline: 0.01725
L-Serine: 0.02625
L-Threonine: 0.05345
L-Tryptophan: Absent
L-Tyrosine�2Na�2H20: 0.05579
L-Valine: 0.05285
Vitamins:
Biotin: 0.0000035
Choline Chloride: 0.00898
Folic Acid: 0.00266
i-inositol: 0.0126
Nicotinamide: 0.00202
D-Pantothenic Acid�Ca: 0.00224
Pyridoxine HCl: 0.000031
Pyridoxal Hydrochloride: 0.002
Riboflavin: 0.000219
Thiamine HCl: 0.00217
Vitamin B12: 0.00068
Other:
D-Glucose: 3.15
Hypoxanthine(Na): 0.0021
Linoleic Acid: 0.000042
Lipoic Acid: 0.000105
Phenol Red: 0.0081
Putrescine�HCl: 0.000081
Pyruvic Acid, Sodium: 0.055
Thymidine: 0.000365
Total: 12.048g/Liter.