Dog IgM elisa kit product blog
Tags: ELISA Kit; Dog IgM; Dog IgM elisa kit;
The Dog Dog IgM n/a (Catalog #MBS564020) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The MBS564020 ELISA Kit recognizes Dog Dog IgM.The Dog IgM n/a product has the following accession number(s) (GI #685030). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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(Or you can also download the PDF Manual for complete product instructions).
Principle of the Assay: The principle of the double antibody sandwich ELISA is represented in Figure 1. In this assay the IgM present in samples reacts with the anti-IgM antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgM antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgM. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3\',5,5\'-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgM in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgM in the test sample. The quantity of IgM in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Samples: Immunoperoxidase Assay for Determination of IgM in Dog Samples
Assay Type: Sandwich. Intended Uses: The total Dog IgM test kits are a highly sensitive two-site enzyme linked immunoassay (ELISA) for measuring IgM in biological samples of Dogs. Dog IgM ELISA Kit or ELISA (enzyme-linked immunosorbant assays) Kits in general, are a valuable research tool for a myriad of applications in a range of scientific settings. Currently, three major types of ELISA formats are used by researchers: sandwich, competitive and indirect. Most commercially available ELISA Kits are sandwich or competitive. Commercially available ELISA Kits contain wells that have been pre-coated with the capture antibody. Please refer to the product manual for the ELISA format of your specific kit. The following patways have been known to be associated with this gene. Dog IgM also interacts with the following gene(s): FLNA. Bone, Brain, Connective Tissue, Ear, Embryonic Tissue, Heart, Kidney, Muscle, Umbilical Cord, Vascular tissues are correlated with this protein.