CytoSelect 96-well Laminin assay kit product blog
Tags: Assay Kit; CytoSelect 96-well Laminin; CytoSelect 96-well Laminin assay kit;
The CytoSelect 96-well Laminin n/a (Catalog #MBS168737) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
(Or you can also download the PDF Manual for complete product instructions).
Please refer to the product datasheet for known applications of a given assay kit. We\'ve tested the CytoSelect 96-well Laminin Cell Invasion Assay, Fluorometric with the following immunoassay(s):
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Principle of the assay: The CytoSelect 96-well Laminin Cell Invasion Assay Kit contains polycarbonate membrane inserts (8 mum pore size) in a 96-well plate. The upper surface of the insert membrane is coated with an uniform layer of dried Murine Laminin I matrix. This laminin matrix layer serves as a barrier to discriminate invasive cells from non-invasive cells. Invasive cells are able to degrade the laminin matrix layer, and ultimately pass through the pores of the polycarbonate membrane. Finally, these invaded cells are then dissociated from the membrane and subsequently detected by the patented CyQuant GR Dye (Invitrogen).
Background: The ability of malignant tumor cells to invade normal surrounding tissue contributes in large part to the significant morbidity and mortality of cancers. Invasiveness requires several distinct cellular functions including adhesion, motility, detachment, and extracellular matrix proteolysis. Metastatic cells produce many proteolytic enzymes (e.g. lysosomal hydrolysates, collagenases, plasminogen activators) while the expression of certain cell surface protease receptors is also increased. CytoSelect 96-well Laminin Cell Invasion Assay Kit utilizes Murine Laminin I-coated inserts to assay the invasive properties of tumor cells. The kit does not require you to prelabel the cells with Calcein AM or remove non-invaded cells (i.e. cotton swabbing). Any invaded cells are first dissociated from the membrane, then lysed and detected with CyQuant GR Dye. CytoSelect 96-well Laminin Cell Invasion Assay Kit provides a robust system for the quantitative determination of cell invasion. It contains sufficient reagents for the evaluation of 96 samples.