Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow CpoI restriction enzyme  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-HAX1 antibody blog
 • Bladder Tissue slide blog
 • SREK1 shrna blog
 • ODF4 sirna blog
 • anti-CD8 antibody blog
 • anti-HLA-DRB1 antibody blog
 • DA elisa kit blog
 • anti-KRT1 antibody blog
 • Plasmin-alpha (α) 2-antiplasmin complex (PAP) elisa kit blog
 • SLC37A3 sirna blog
 • anti-CD134L/OX40L antibody blog
 • anti-IFNA1 antibody blog
 • ST3GAL4 shrna blog
 • GLRB blocking peptide blog
 • PARK2 sirna blog
 • Tissue, Total Protein, Hamster Adult Normal, Liver lysate blog
 • Tissue Section Disease, Parkinson, Brain, Pons tissue section blog
 • CD4 blocking peptide blog
 • anti-SCUBE1 antibody blog
 • TGIF2 peptide blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Histone Antibody (AHA) ELISA Kit
 • OPN Antibody
 • papillomavirus type 16 Protein E6 Antibody
 • gonadotropin-releasing hormone 1 (luteinizing-releasing horm... ELISA Kit
 • A disintegrin and metalloproteinase with thrombospondin moti... ELISA Kit
 • MYST histone acetyltransferase (monocytic leukemia) 4 (MYST4... ELISA Kit
 • I-309 (I 309) Recombinant Protein
 • Prostaglandin E2 (PG-E2) ELISA Kit
 • Thymidylate kinase (tmk) Recombinant Protein
 • Major Basic Protein (MBP) Antibody
 • GABAA Receptor a 4, (GABRA4) Antibody
 • Parathyroid hormone (PTH) ELISA Kit
 • SNAPC4 Antibody
 • Plexin B2 (PLXNB2) ELISA Kit
 downarrow more ...

CpoI restriction enzyme product blog

Posted on 2017-05-29 04:47:23 by mybiosource_staff
Share it on ...     
Tags: Restriction Enzyme; CpoI; CpoI restriction enzyme;
The CpoI n/a (Catalog #MBS638374) is a Restriction Enzyme produced from Caseobacter polymorphus and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


CpoI n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Compatible Ends: Cfr13I, Eco47I, Psp5II, SanDI
pACYC177: 0
pACYC184: 0
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda: 5
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with CpoI, more than 95% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.05uM. More than 95% of these can be recut.
M13mp18/19: 0
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with CpoI.
pBR322: 0
PhiX174: 0
pTZ19R/U: 0
pUC18/19: 0
pUC57: 0
Stability During Prolonged Incubation: A minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions