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CpoI restriction enzyme product blog

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Posted on 2017-05-29 04:47:23 by mybiosource_staff
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Tags: Restriction Enzyme; CpoI; CpoI restriction enzyme;
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The CpoI n/a (Catalog #MBS638374) is a Restriction Enzyme produced from Caseobacter polymorphus and is intended for research purposes only. The product is available for immediate purchase.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


T.

CpoI n/a

Dilution Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol.
Compatible Ends: Cfr13I, Eco47I, Psp5II, SanDI
pACYC177: 0
pACYC184: 0
Blue/White Cloning Assay: The mix of pUC57/HindIII, pUC57/Eco32I and pUC57/PstI digests was incubated with 10units of enzyme for 16 hours. After religation and transformation 0.3% of white colonies were detected. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda: 5
Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with CpoI, more than 95% of the DNA fragments can be ligated at a 5\'-termini concentration of 0.05uM. More than 95% of these can be recut.
M13mp18/19: 0
Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with CpoI.
pBR322: 0
PhiX174: 0
pTZ19R/U: 0
pUC18/19: 0
pUC57: 0
Stability During Prolonged Incubation: A minimum of 0.5units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37 degree C.
Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation: Enzyme is inactivated by incubation at 65 degree C for 20min.
Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37 degree C in 50ul of assay buffer.
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