Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Blog  rightarrow CMV antigen  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • MMP13 blocking peptide blog
 • hsa-miR-652-5p mirna blog
 • MGAT4A sirna blog
 • ANN-II elisa kit blog
 • PLA2G4D blocking peptide blog
 • ITGB5 blocking peptide blog
 • PRRT2 sirna blog
 • LRIG2 sirna blog
 • anti-SHPRH antibody blog
 • ZNF615 sirna blog
 • CCR3 sirna blog
 • anti-CD98 antibody blog
 • PPP1R18 shrna blog
 • HRP Rabbit Anti-Camel IgG (H+L) secondary antibody blog
 • anti-NA antibody blog
 • EVL peptide blog
 • ARSE shrna blog
 • Tissue, Genomic DNA Disease genomic dna blog
 • HSPG2 elisa kit blog
 • anti-THRA antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Interleukin-15 (IL15) Recombinant Protein
 • Aquaporin 9 (AQP-9) ELISA Kit
 • Zika Virus Envelope Protein E Antibody
 • MIP-1 ALPHA Recombinant Protein
 • Adalimumab (Humira) PK ELISA Kit
 • Insulin Like Growth Factor1 (IGF1) ELISA Kit
 • Mycoplasma pneumoniae Antibody
 • Histamine ELISA Kit
 • CaMK2-beta- gamma- delta Antibody
 • C-peptide ELISA Kit
 • N-Methyl-D-Aspartate (NMDA) Receptor 2 (NR2) (LOC100533244) ELISA Kit
 • Apical membrane antigen 1 (AMA-1), partial Recombinant Protein
 • early growth response 1 (EGR1) ELISA Kit
 downarrow more ...

CMV antigen product blog

Posted on 2013-08-27 13:52:31 by mybiosource_staff
Share it on ...     
Tags: CMV; Antigen; Cytomegalovirus (CMV) Antigen; CMV antigen;
The CMV n/a (Catalog #MBS318657) is an Antigen produced from HF Cells and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Cytomegalovirus (CMV) Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Researchers should empirically determine the suitability of the CMV n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Viral Strain: AD169
Propagated in HF Cells

Introduction: Cytomegalovirus (CMV) antigen is considered one of the most common viral infections in man. The virus rarely causes disease, but may persist as a latent or chronic infection of the salivary glands, renal tubules, cervix and leukocytes. CMV infections are frequent and occasionally severe in children or adults with congenital or acquired defects of cellular immunity, such as patients with AIDS, cancer and recipients of organ transplants. The transmission of CMV via transfused blood may account for the majority of the CMV infections in organ transplant recipients, neonates and heart surgery patients. Medical conditions resulting from Cytomegalovirus infections include: interstitial pneumonia, mononucleosis (heterophile negative), congenital defects, abortion, mental retardation, blindness and deafness.
We supplies a variety of Cytomegalovirus (CMV) antigens: CMV, CMV-M, CMV-EXT 2 and our new CMV Grade III. All antigens are propagated in human fibroblast cells and infected with the viral strain, AD169. The antigen preparations are partially purified to reduce host cell components prior to inactivation by the extraction process or ultraviolet light yielding high sensitivity and low background antigens useful in a variety of solid phase immunoassays.
The various CMV antigens are designed by the extraction process to perform best in either an IgG or an IgM solid phase immunoassay but several of the antigens can be used for both IgG and IgM depending on the application.
CMV-M antigens are available at a range of concentrations: #7511 (0.3 - 0.4 mg/mL) and #7090 (~1.0mg/mL). The CMV-M antigen process extracts antigens primarily from the nucleus and endoplasmic reticulum of the infected cells to enhance the amount of antigens reacting with the early IgM antibody. In contrast, the entire cell is extracted for the #7504 CMV (0.2 - 0.3 mg/mL) antigen.
The CMV EXT2 process for #7517 is designed to select the CMV glycoprotein antigens of the infected fibroblast cells. This enriches for the antigens found on the surface of the cell that react with IgG antibodies.
The new CMV Grade III antigen #7507 is purified by column chromatography to enrich for pp65 along with other CMV antigens, see the graphic below. The chromatography purified CMV Grade III has a higher specific activity after the removal of impurities and strong reactivity for both IgG and IgM sera.

CMV n/a

Source: Grown in Human Fibroblast Cells
Product manufactures in the United States using only domestic animal materials processed in U.S facilities). Activity: ELISA Assay: Reference Lot Number: M16961010
Testing Results: Midpoint Titer (test antigen): 840.91
% of Reference: 10.15%

Testing Results: Midpoint Titer (reference antigen): 763.456

Background ELISA Ratio: Aeceptable:< or = 2
Test Result: 1.951

Sensitivity/Specificity Medium Positive Control: Acceptable > or = 3
Test Result: 24.849

Sensitivity/Specificity High Positive Control: Acceptable > or = 8
Test Result: 35.151
Buffer: 0.1 M Glycine Buffer, pH 9.3 9.7
Preservative: None
Inactivation: Product inactivated by chemical disruption.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions