|The CMV n/a (Catalog #MBS318657) is an Antigen produced from HF Cells and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s Cytomegalovirus (CMV) Antigen can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Researchers should empirically determine the suitability of the CMV n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Viral Strain: AD169
Propagated in HF Cells
Introduction: Cytomegalovirus (CMV) antigen is considered one of the most common viral infections in man. The virus rarely causes disease, but may persist as a latent or chronic infection of the salivary glands, renal tubules, cervix and leukocytes. CMV infections are frequent and occasionally severe in children or adults with congenital or acquired defects of cellular immunity, such as patients with AIDS, cancer and recipients of organ transplants. The transmission of CMV via transfused blood may account for the majority of the CMV infections in organ transplant recipients, neonates and heart surgery patients. Medical conditions resulting from Cytomegalovirus infections include: interstitial pneumonia, mononucleosis (heterophile negative), congenital defects, abortion, mental retardation, blindness and deafness.
We supplies a variety of Cytomegalovirus (CMV) antigens: CMV, CMV-M, CMV-EXT 2 and our new CMV Grade III. All antigens are propagated in human fibroblast cells and infected with the viral strain, AD169. The antigen preparations are partially purified to reduce host cell components prior to inactivation by the extraction process or ultraviolet light yielding high sensitivity and low background antigens useful in a variety of solid phase immunoassays.
The various CMV antigens are designed by the extraction process to perform best in either an IgG or an IgM solid phase immunoassay but several of the antigens can be used for both IgG and IgM depending on the application.
CMV-M antigens are available at a range of concentrations: #7511 (0.3 - 0.4 mg/mL) and #7090 (~1.0mg/mL). The CMV-M antigen process extracts antigens primarily from the nucleus and endoplasmic reticulum of the infected cells to enhance the amount of antigens reacting with the early IgM antibody. In contrast, the entire cell is extracted for the #7504 CMV (0.2 - 0.3 mg/mL) antigen.
The CMV EXT2 process for #7517 is designed to select the CMV glycoprotein antigens of the infected fibroblast cells. This enriches for the antigens found on the surface of the cell that react with IgG antibodies.
The new CMV Grade III antigen #7507 is purified by column chromatography to enrich for pp65 along with other CMV antigens, see the graphic below. The chromatography purified CMV Grade III has a higher specific activity after the removal of impurities and strong reactivity for both IgG and IgM sera.
Source: Grown in Human Fibroblast Cells
Product manufactures in the United States using only domestic animal materials processed in U.S facilities). Activity: ELISA Assay: Reference Lot Number: M16961010
Testing Results: Midpoint Titer (test antigen): 840.91
% of Reference: 10.15%
Testing Results: Midpoint Titer (reference antigen): 763.456
Background ELISA Ratio: Aeceptable:< or = 2
Test Result: 1.951
Sensitivity/Specificity Medium Positive Control: Acceptable > or = 3
Test Result: 24.849
Sensitivity/Specificity High Positive Control: Acceptable > or = 8
Test Result: 35.151
Buffer: 0.1 M Glycine Buffer, pH 9.3 9.7
Inactivation: Product inactivated by chemical disruption.